Vivantis Technologies Sdn Bhd

Concentration
20-40u/μl

5'…GR↓CGYC…3'
3'…CYGC↑RG…5'

Reaction Conditions
1X Buffer V4
10mM Tris-HCl (pH8.5 at 30°C), 10mM MgCl₂, 100mM KCl and 100µg/ml BSA.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay
After 20-fold overdigestion with BssNI, 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of BssNI for 16 hours at 37°C.

Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Download
Manual
BssNI {AcyI}

Download
MSDS
BssNI {AcyI}