
PCR reaction was performed in 25 μl final volume that contained 20.75 μl PCR master mix (Vivantis, Cat # PLMM01), 0.25 μl Taq polymerase (Vivantis, Cat #PL1202 or #PL1204) in a research involved differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1 by a group of searchers from Tehran, Iran.
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