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GF-1 Bacterial DNA Extraction Kit

Description
The GF-1 Bacterial DNA Extraction Kit provides a rapid and efficient method for purification of high molecular weight genomic DNA from either Gram-negative or Gram-positive bacteria. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance the binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA.

Features

  • Suitable for both Gram-negative or Gram-positive bacteria
  • Yields up to 20μg of DNA
  • No organic-based extraction required
  • Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern blotting and DNA fingerprinting.

Kit Components

  • Buffer R1
  • Buffer R2
  • Buffer BG
  • Wash Buffer (concentrate)
  • Elution Buffer
  • Proteinase K

Ordering Information

Catalog No Description Pack Size
GF-BA-050 GF-1 Bacterial DNA Extraction Kit 50 preps
GF-BA-100 GF-1 Bacterial DNA Extraction Kit 100 preps

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GF-1 Bacterial DNA Extraction Kit

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GF-1 Bacterial DNA Extraction Kit

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GF-1 Bacterial DNA Extraction Kit

Publication
This Product Has Been Used In:

Seleim S M et al. (2022) The role of pmrCAB genes in colistin‑resistant Acinetobacter baumannii. Scientific reports. 12:20951

Thi-Hoang-Nga-Vo et al. (2022) Multidrug Resistance and Serotype Distribution of Salmonella Enterica Isolated from Homemade Recipe Fermented Ground Pork (Nham) in Northeastern Thailand, Trends in Sciences , Vol 19 No.1

Hamid THTA & Fuzi NFAM (2021) Lactic acid bacterium with antimicrobial properties from selected Malay traditional fermented foods, International Journal of Life Sciences and Biotechnology.

Karray F et al. (2021) Production of Poly(3-Hydroxybutyrate) by Haloarcula, Halorubrum and Natrinema Haloarchaeal genera using starch as a carbon source, Archaea, 1-10.

Nahed HG et al. (2021) Isolation and molecular characterization of Campylobacter jejuni from chicken and human stool samples in Egypt, Food Science and Technology, 41(1):195-202.

Vella A et al. (2021) Resurrection of the Butterfly-winged comber, Serranus papilionaceus Valenciennes, 1832 (Teleostei, Serranidae) and its phylogenetic position within genus Serranus, ZooKeys, 1017:111-126.

Ekprasert J et al. (2020) Investigating mechanical properties and biocement application of CaCO3, precipitated by a newly-isolated Lysinibacillus sp. WH using artificial neural networks, Scientific Reports,10:16137.

Erkihun A & Kausalya R (2020) MCR-1 Gene Encoded Colistin-Resistant Escherichia coli in Raw Chicken Meat and Bean Sprouts in Malaysia, International Journal of Microbiology, Article ID 8853582.

Eshamah HL et al. (2020) Extent of pathogenic and spoilage microorganisms in whole muscle meat, meat products and seafood sold in Libyan market, Open Veterinary Journal,10(3):276-288.

Fatin Aina Zulkhairi Amin et al. (2020). Probiotic Properties of Bacillus Strains Isolated from Stingless Bee (Heterotrigona itama) Honey Collected across Malaysia,. Int. J. Environ. Res. Public Health, 17, 278.

Nonzee H et al. (2020) Interaction Study of Pasteurella Multocida with Culturable Aerobic Bacteria Isolated from Porcine Respiratory Tracts using Coculture in Conditioned Media, Research Square,https://doi.org/10.21203/rs.3.rs-36993/v1

John Paul MDG et al. (2020) Green tea (Camelia sinensis) extract inhibits biofilm formation in acyl homoserine lactone-producing, antibiotic-resistant Morganella morganii isolated from Pasig River, Philippines, Heliyon, 6(2020) e05284.

Pongrawee N & Neelawan P (2020) Distribution and expression of virulence genes in potentially pathogenic bacteria isolated from seafood in Thailand, CyTA – Journal of Food 18:1, 753-763.

Virun Vichaibun & Panan Kanchanaphum (2020) Quantitative LAMP and PCR Detection of Salmonella in Chicken Samples Collected from Local Markets around Pathum Thani Province, Thailand, International Journal of Food Science, Article ID 8833173.

in vitro antimicrobial assessment on lactic acid bacteria isolated from common freshwater fishes,. Asia-Pacific Journal of Molecular Biology and Biotechnology, 27(2):18-25.

Mankai H et al (2019) Complete Genome Sequence and Methylome Analysis of Thermoactinomyces vulgaris 2H, . Microbiology Resource Announcements, 8:e00657-19.

Tung HJ et al. (2019) cDNA-SSR Markers for Molecular Epidemiology of Ganoderma boninense,. , Journal of Oil Palm Research, 31(2):220-237.

Boonyayatra, S., Tharavichitkut, P., Oliver, S.P. (2018). Virulence-associated genes and molecular typing of Streptococcus uberis associated with bovine mastitis in northern Thailand, Turkish Journal of Veterinary and Animal Sciences, Vol. 42, 73-81 (2018).

Cilingir,et al. (2018). The Relationship between Glutathione-S-Transferases Polymorphisms and Lichen Planus Susceptibility. International Journal of Research Studies in Medical and Health Sciences Volume 3, Issue 1, 2018, PP 15-22

Intrakamhaeng M et. al. (2018) The occurrence of MRSA, MSSA and antibiotic resistance, related factors in area of dairy farming of Mahasarakham province, Thailand, International Journal of Agricultural Technology 2018 Vol. 14(7): 1259-1266

Ashraf, A. Abd El Tawwab et. al (2017). The use of multiplex PCR for Identification of Mycobacterium bovis isolates in Egypt. Nature and Science 2017;15(8)

Can, H.Y., Elmali, M., Ergün, Y. (2017). Methicillin-resistant Staphylococcus aureus in milk from dairy cows with chronic mastitis, Eurasian Journal of Veterinary Sciences, Vol. 33, No. 4, 255-259 (2017).

Daniel, D.S., Gan, H.M., Lee, S.M., Dykes, G.A., Rahman, S. (2017). Draft Genome Sequences of Six Enterococcus faecalis Strains Isolated from Malaysian Clinical and Environmental Origins, , Genome Announcements, Vol. 5, No. 24.

Daniel, D.S., Lee, S.M., Gan, H.M., Dykes, G.A., Rahman, S. (2017) )Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia, . Journal of Infection and Public Health Vol. 10, No. 5, 617-623.

Elmali, M., Can, H.Y. (2017). Occurence and antimicrobial resistance of Arcobacter species in food and slaughterhouse samples, , Journal of Food Science and Technology, Vol. 37, No. 2, 280-285 (2017).

Fibriana, F., Amalia, A.V., Mubarok,I. (2017). )Isolation and Characterization of Pigment Generating Microorganisms from Potato Skin. . Jurnal Mipa, Vol. 40, No. 1, 7-13 (2017).

Guran, H.S., Vural, A., Erkan, M.E., Durmusoglu. (2017) Prevalence and some virulence genes of E. coli O157 isolated from chicken meats and giblets Ann. Anim. Sci., Vol. 17, No. 2 (2017) 555–563

Idrees, H.A., Omer, A.F.A., Elaas, W.A., Mohammed, Z.M.S., Sidig, M., (2017) Serological Significance of Anti-HBc IgG Marker detected in Sudanese Haemodialysis Patients . American Journal of Research Communication2017, 5(8): 1-8.

Wilantho, A., Deekaew, P., Srissutti, C., Tongisma, S., Somboonna, N. (2017). Diversity of bacterial communities on the facial skin of different age-group Thai males PeerJ, (2017).

Zakaria, N.H., Tajarudin, H.A., Sapingi, M.S.M., Murshed, M.F. (2017). Identification of Pathogenic Bacteria Isolated from Raw and After Sand Filtration Water at Lubok Buntar Water Treatment Plant, Scientific Research Journal, Vol. 14, No. 1 (2017).

Azwai, S.M. et al. (2016) Isolation and Molecular Identification of Vibrio spp. By sequencing of 16S rDNA from seafood, meat and meat products in Libya. Open Veterinary Journal, 6(1), p. 36-43.

Aw, Y.K., et al (2016) Newly Isolated Paenibacillus tyrfis sp. nov., from Malaysian Tropical Peat Swamp Soil with Broad Spectrum Antimicrobial Activity. Frontiers in Microbiology. 7(219), p. 1-9.

Copur-Cicek,A., Dinc-Patlak, R., Sandalli, C., Mengeloğlu, Z., Kostakoglu, U., Yildiz, I.E. (2016). Determination of the staphylococcal cassette chromosome in methicillin-resistant Staphylococcus aureus strains isolated from various clinical samples. . Russia Open Medical Journal. 5(2).

Luang-In,V., Deeseenthum, S. (2016) Exopolysaccharide-producing isolates from Thai milk kefir and their antioxidant activities. . LWT - Food Science and Technology.73. Pp592-601

Merghani, E.A.E., Elsanousi, S.M., Hamedelnil, Y.F., Gornas, N.A. (2016). Molecular Study of Bacterial Pathogens Isolated From Haemodialysis Patients, Sudan Journal of Science and Technology, Vol 12, No. 1, 1-10 (2016).

Mohamad, Y., Reda, W.W., Abdel-Moein, K., El-Razil, K.A.A., Barakat, A.M.A., El Fadaly, H.A., Hassanain, N.A., Hegazi, A.G. (2016) Prevalence and phylogenetic characterization of Listeria Monocytogenes isolated from processed meat marketed in Egypt.. Journal of Genetic Engineering and Biotechnology. 14(1). Pp.119-123

Raweekul, W., Wuttitummaporn, S., Sodchuen, W., Kittiwongwattana, C. (2016). Plant Growth Promotion by Endophytic Bacteria Isolated from Rice (Oryza sativa), Thammasat International Journal of Science and Technology,Vol. 21, No. 1 (2016).

Supong, K., Thawai, C., Supothina, S., Auncharoen, P., Pittayakhajonwut, P. (2016) ) Antimicrobial and anti-oxidant activities of quinoline alkaloids from Pseudomonas aeruginosa BCC76810. Phytochemistry Letters. 17. Pp.100-106

Uttatree,S., Charoenpanich, J. (2016) Isolation and characterization of a broad pH- and temperature-active, solvent and surfactant stable protease from a new strain of Bacillus subtilis. . Biocatalysis and Agricultural Biotechnology. 8. Pp.32-38

Abdulrahman, S.M. (2015). Multiplex PCR for direct identification of Campylobacter Species in Human Stool Journal of Biomedical and Pharmaceutical Research, Vol. 4, No. 3 (2015).

Akinsaya, M. A. et al. (2015) Metagenomics Study of Endophytic Bacteria in Aloe Vera Using Next Generation Technology. Genomics Data. ScienceDirect6, p. 159-163.

Chua, P., Har, Z.M., Austin, C.M., Yule, C.N., Dykes, G.A., Lee, S.M. (2015) Genome sequencing and annotation of Aeromonas sp. HZ. . Genomics Data. 5 pp38-39

Chua, P., Har, Z.M., Austin, C.M., Yule, C.N., Dykes, G.A., Lee, S.M. (2015) Genome sequencing and annotation of Cellulomonas sp. HZM. . Genomics Data.5. Pp40-41

Erdogmus, S.F., et al (2015) Aromatic Hydrocarbon Utilization Ability of Chromohalobacter sp. Ekoloji94: 10-16.

Khalafalla, A.I., Al-Busada, K.A., & El-Sabagh, I.M. (2015) Multiplex PCR for Rapid Diagnosis and Differentiation of Pox and Pox-like Diseases in Dromedary Camels. Virology Journal. 12(102), p. 1-10.

Ntougias, S., Melidis, P., Navrozidou, E., Tzegkas, F. (2015) Diversity and efficiency of anthracene-degrading bacteria isolated from a denitrifying activated sludge system treating municipal wastewater. International Biodeterioration & Biodegradation. 97. Pp..151-158.

Abd El-Salam, N.M., Ayaz, S., & Ullah, R. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa. BioMed Research International.

Abd El-Salam, N.M., Ayaz, S., & Ullah, R. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa. BioMed Research International.

Chua, P., et al. (2014) Draft Genone Sequences of Two Cellulolytic Paenibacillus sp. Strains, MAEPY1 and MAEPY2, from Malaysian Landfill Leachate. Genone Announcements.2(1).

El-Salam et al. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa Biomed Res Int92014: 861831.

Heng, J.L.S., & Hamzah, H. (2014) Biological Active Compounds from Actinomycetes Isolated from Soil of Langkawi Island, Malaysia. African Journal of Biotechnology.ProQuest. 13(49), p. 4523-4528

Rahama, A.B.M., et. al (2014) Molecular Detection of helicobacter pylori Among Gastroduodenitis and Peptic Ulcer Patients in Khartoum State. Journal of Biomedical and Pharmaceutical Research3(5): 41-44

Ting, A.S.Y., Hermanto, A., Peh, K.L. (2014) Indigenous actinomycetes from empty fruit bunch compost of oil palm: Evaluation on enzymatic and antagonistic properties. Biocatalysis and Agricultural Biotechnology. 3. Pp..310-315.

Wanangkarn, A., Liu, D.C., Swetwiwathana, A., Jindaprasert, A., Phraephaisarn, C., Chumnqoen, W., Tan, F.J. (2014) Lactic acid bacterial population dynamics during fermentation and storage of Thai fermented sausage according to restriction fragment length polymorphism analysis. International Journal of Food Microbiology. 186. Pp..61-67

Erdomus, S.F., et al. (2013) Aromatic Hydrocarbon Degradation by Halophilic Archaea Isolated from Camalti Saltern, Turkey Water, Air and Soil Pollution. ProQuest. p. 1-9.

Hafshejani, M.K., et al (2013)Sequential Microaerophilic-Oxic Phase Mineralization of Azo Dyes by a Monoculture of Pseudomonas Aeruginosa Strain AWF Isolated from Textile Wastewater Water Air and Soil Pollution. ProQuest. p. 1-16.

Naher, U.A., Othman, R., Abdul-Latif, M., Panhwar, Q.A., Amaddin, P.A.M., Shamsuddin, Z.H. (2013). Biomolecular Characterization of Diazotrophs Isolated from the Tropical Soil in Malaysia, International Journal of Molecular Sciences, Vol. 14, 17812-17829 (2013).

Gumel et al.(2012)Biosynthesis and Characterization of Polyhydroxyalkanoates Copolymers Produced by Pseudomonas putida Bet001 Isolated from Palm Oil Mill Effluent PLoS One7(9).

Pongsilp, N., et al (2012) Genotypic Diversity among Rhizospheric Bacteria of Three Legumes Assessed by Cultivation-dependent and Cultivation-independent Techniques World Journal of Microbiology and Biotechnology. ProQuest. 28, p. 615-626.

Shamsudin, M.N., et al (2012)High Prevalence of qacA/B Carriage among Clinical Isolates of Meticillin-resistant Staphylococcus aureus in Malaysia Journal of Hospital Infection81: 206

Charoenpanich, J., Suktanarag, S., & Toobbucha, N. (2011)Production of Thermostable Lipase by Aeromonas sp. EBB-1 Isolated from Marine Sludge in Angsila, Thailand ScienceAsia37: 105-114.

Mat Hussin, N. H. et al (2011)Characterization of Bacteria and Fungi Bioaerosol in the Indoor Air of Selected Primary Schools in Malaysia Indoor and Built Environment. Sage Journals20(6), p. 607-617

Nurul Zarith Mohamad Zin et. al. (2011) Characterization and antimicrobial activities of two Streptomyces isolates from soil in the periphery of Universiti Putra Malaysia. Tropical Biomedicine 28(3): 651–660 (2011)

Atshan, S.S., & Shamsudin, M.N. (2010)Evaluation of Phenotypic and Genotypic Detection Methods for Biofilm-forming Methicillin-sensitive and Methicillin-resistant Staphylococcus aureus Clinical Isolates Annals of Microbiology.

Bayram, Banu et al. (2010). Polycystic ovary syndrome and MTHFR gene C677T and A1298C polymorphisms. Fabad Journal of Pharmaceutical Sciences. 35. 13-17.

Uttatree, S., Winayanuwattikun, P., & Charoenpanich, J. (2010) Isolation and Characterization of a Novel Thermophilic-organic Solvent Stable Lipase from Acinetobacter baylyi Applied Biochemistry and Biotechnology

Zakaria, M.R., et al. (2010)Polyhydroxyalkanoate Production from Anaerobically Treated Palm Oil Mill Effluent by New Bacterial Strain Comamonas sp. EB172 World Journal of Microbiology and Biotechnology. ProQuest. 26, p. 767-774.

 

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