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Home ViPrimePLUS Real-Time PCR Kits Human Infection qPCR/RT-qPCR Kits Virus ViPrimePLUS 2019-nCoV Multiplex RT-qPCR Kit

ViPrimePLUS 2019-nCoV Multiplex RT-qPCR Kit

  • Multiplex detection in a single tube: N gene, RdRP gene and E gene
  • Ready-to-use solution with all necessary components (primer, probe and master mix) in a single tube
  • Positive control with internal control: human gene is included
  • Minimize technical error
  • Enable immediate reaction set up with short processing time
  • Validation report available

Kit Component

  • 2019-nCoV Primers and Probe Mix (2019-nCoV PPM)
  • Positive Control
  • Nuclease Free Water
  • ROX Dye (Low ROX)
  • ROX Dye (High ROX)

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit RNA
Kit Storage Shipped and stored at -20℃
Sample Material Clinical samples
- Nasopharyngeal and oropharyngeal swab soaked in VTM buffers;
- Whole blood, serum and plasma of infected patients
- COVID-19 cell culture supernatant
Gene Target 2019-nCoV genome:
- Nucleoprotein, N gene
- RNA-dependent RNA polymerase, RdRP gene
- Envelope small membrane protein, E gene
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Positive control
Channels • FAM channel detects N gene
• VIC/HEX channel detects Human gene (IC)
• TEXAS RED channel detects RdRP gene
• CY5 channel detects E gene

Graph

N gene (FAM channel)

 

RdRP gene (Texas Red channel)

 


E gene (Cy5 channel)

 

Figure: The graphs show the sensitivity of the kit performance for different targeted genes in ViPrimePLUS 2019-nCoV Multiplex RT-qPCR Kit. The kit was tested for sensitivity with different quantity of RNA copies range from 1,000,000 copies to 10 copies. The graphs and CT values show that the kit can detect minimum 10 copies of RNA copies for N gene and E gene; detect minimum 100 copies of RNA copies for RdRP gene.


Ordering Information

Catalog No Description Pack Size
QM50811 ViPrimePLUS 2019-nCoV Multiplex RT-qPCR Kit 100 reactions

 

Download
Manual

ViPrimePLUS 2019-nCoV Multiplex RT-qPCR Kit


Application Note

COVID-19 Testing on Saliva Sample using GF-1 Viral Nucleic Acid Extraction Kit and ViPrimePLUS 2019-nCoV Multiplex RT-qPCR Kit

 

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