16 March 2017
Viva cDNA Synthesis Kit is specially designed to provide reliable synthesis of full-length cDNA. M-MuLV RNase synthesizes complementary DNA strand initiating from a specific primer, oligo d(T) or random hexamer. The absence of RNase H enhances the synthesis of long cDNA as the RNA strand does not degrade in DNA-RNA hybrid during first strand cDNA synthesis. This cDNA synthesis kit is readily compatible with various cDNA-dependent downstream applications.
Features:
- Absence of RNase H activity allows high yield of full length
- cDNA synthesis with RNA templates up to 10kb
- Wide selection of primers, oligo d(T) or random hexamer
- Highly compatible with various downstream applications
- Allows synthesis of full length cDNA from various RNA templates
- High capacity and able to copy up to 2μg of purified mRNA
- Traditional PCR amplification or real-time PCR quantification
- Storage of selective and functional genetic information by removing of introns
- Cloning of the gene of interested enables horizontal gene transfer (HGT)
- cDNA probe library construction with large and full-length inserts
- cDNA microarray for gene discovery and clinical diagnosis via gene pattern screening
- mRNA expression studies and storage of proteins material at genomics level
