Vivantis Technologies Sdn Bhd

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Apa I

Concentration
20u/μl

5 ' . . . G G G C C↓C . . . 3 '
3 ' . . . C↑C C G G G . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

Unit Definition
20u (1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation
65˚C for 20 minutes.

Quality Control Assays
Ligation/ Recutting Assay

After 20-fold overdigestion with Apa I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of Apa I for 16 hours at 37˚C.

Ordering Information

Catalog No Description Pack Size
FDRE1124 Apa I 50 preps

 

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Apa I

 

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