Vivantis Technologies Sdn Bhd

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EcoR I

Concentration
20u/μl

5 ' . . . G↓A AT T C . . . 3 '
3 ' . . . C T TA A↑G . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

Unit Definition
20u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation
65˚C for 20 minutes.

Quality Control Assays
Ligation/ Recutting Assay

After 20-fold overdigestion with EcoR I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of EcoR I for 16 hours at 37˚C.

Ordering Information

Catalog No Description Pack Size
FDRE1260 EcoR I 100 preps

 

Downloads

Manual

EcoR I

Publication
This Product Has Been Used In:

Siti-Hasmah, M., Hwe-San, L., Xiao-Ying, C., Massawe, F., Abdul-Rahman, O. (2017). Single Nucleotide Modification of VP2 Gene of Highly Virulent Infectious Bursal Disease Virus for Effective Expression Strategy in Tobacco. . Open Access Journal of Microbiology & Biotechnology, Vol. 2, No. 1 (2017).

 

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ADDITIONAL INFORMATION

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Reaching Us

Headquarters

Vivantis Technologies Sdn Bhd

Revongen Corporation Center,

Level 17, Top Glove Tower,

No. 16, Persiaran Setia Dagang,

Setia Alam, Seksyen U13,

40170 Shah Alam,

Selangor Darul Ehsan,

Malaysia.


Tel: +603-3359 1166

Fax: +603-3358 0303

 

 

ACCOLADES & ACCREDITATION

Acquaint Us

International Distribution/OEM services:

Email: international@vivantechnologies.com

WhatsApp: +6016-216 0160


Local Distribution/Sales Inquiries:

Email: info@vivantechnologies.com

WhatsApp: +6016-627 2323


Product Technical Support:

vivalab@vivantechnologies.com


General Enquiries:

info@vivantechnologies.com

 

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