Vivantis Technologies Sdn Bhd

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EcoR I

Concentration
20u/μl

5 ' . . . G↓A AT T C . . . 3 '
3 ' . . . C T TA A↑G . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

Unit Definition
20u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation
65˚C for 20 minutes.

Quality Control Assays
Ligation/ Recutting Assay

After 20-fold overdigestion with EcoR I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of EcoR I for 16 hours at 37˚C.

Ordering Information

Catalog No Description Pack Size
FDRE1260 EcoR I 100 preps

 

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EcoR I

Publication
This Product Has Been Used In:

Siti-Hasmah, M., Hwe-San, L., Xiao-Ying, C., Massawe, F., Abdul-Rahman, O. (2017). Single Nucleotide Modification of VP2 Gene of Highly Virulent Infectious Bursal Disease Virus for Effective Expression Strategy in Tobacco. . Open Access Journal of Microbiology & Biotechnology, Vol. 2, No. 1 (2017).

 

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