22 Dec 2016 (Malaysia) - Good bye to cold-shipping Proteinase K with the new Proteinase K from Vivantis. From January 2017, Vivantis will be replacing Proteinase K with better specification as below.
Change of Specification of Proteinase K (Aqueous Solution Form)
| Changes | Current Specification | New Specification |
|---|---|---|
| Form of enzyme | Native protease | Recombinant protease |
| Storage condition | -20°C | 2 - 8°C |
| Shipping condition | Shipped in cold condition | Shipped in room temperature condition |
Ordering Information
| Catalogue No. | Description | Pack Size |
|---|---|---|
| PC0712-1ml | Proteinase K | 1ml |
Change of Specification of Proteinase K (Lyophilized Form)
| Changes | Current Specification | New Specification |
|---|---|---|
| Form of enzyme | Native protease | Recombinant protease |
| Storage condition | -20°C | Room temperature |
| Shipping condition | Shipped in cold condition | Shipped in room temperature condition |
Ordering Information
| Catalogue No. | Description | Pack Size |
|---|---|---|
| PC0712-100mg | Proteinase K | 100mg |
| PC0712-500mg | Proteinase K | 500mg |
| PC0712-1g | Proteinase K | 1g |
Proteinase K is a subtlisin-related serine protease originally isolated from the mold, Tritirachium album. Vivantis new Proteinase K is a recombinant enzyme. The key specifications of the recombinant enzyme are the same as those of the native protease with the amino acid sequence and the molecular structure are identical. Proteinase K hydrolyzes a variety of peptide bonds in a wide range of temperatures and buffers with optimal activity between 20 - 60°C and a pH between 7.5 and 12.0. Proteinase K is also active in buffers containing chelating agents such as EDTA.
The recombinant preparation is much purer than the native enzyme. In particular, since Proteinase K, is DNA-free, it is especially suitable for isolating PCR and RT-PCR templates.
Concentration
20-22mg/ml in 10mM Tris-HCl, pH7.5. The solution contains calcium acetate as stabilizer.
Storage and stability
The undiluted solution is stable at 2 - 8°C. Shipment condition can be room temperature.
Volume activity
50U/mL. One unit is the enzyme activity which cleaves at 25°C in 1 minute 18mmol Chromozym TRY (equivalent to 600U/ml with the haemoglobin assay.)
Specific activity
Approximately 2.5U/mg when assayed with the chromozym assay; equivalent to 30U/mg with the haemoglobin assay
Cleavage specificity
Proteinase K is one of the most active endopeptidases known and does not show any pronounced cleavage specificity. If excess proteinase K is incubated with proteins for a long time, the enzyme will degrade the proteins to free amino acids.
Optimum activity
| Stable pH range | pH 4 to pH 12.5. |
|---|---|
| Activity pH range | pH 6.5 to pH 9.5 |
| Optimum temperature | 65°C |
| Denaturation temperature | above 65°C |
| Reaction-active buffers | buffers containing denaturing reagents such as urea, sodium dodecyl sulphate (SDS), and guanidinium salts |
Applications
- Digest native proteins
- Inactivate endogenous RNases and DNases and enzymes effectively
- Isolation of native RNA and DNA from tissues or cell lines
- Promote cell lysis by activating a bacterial autolytic factor
- Analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces by modifying proteins and glycoproteins on cell surfaces.
- Isolating PCR and RT-PCR templates.
- Removes cell debris to improve cloning efficiency
- Treat tissue sections to ensure efficient probe infiltration during in situ hybridization
