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Home Biochemical Biochemicals 3-[(3-cholamidopropyl)dimethyl-ammonio]-1- propanesulfonate (CHAPS)

3-[(3-cholamidopropyl)dimethyl-ammonio]-1- propanesulfonate (CHAPS)

Grade
Ultra Pure Grade

Description
CHAPS is a standard reagent suitable for use as a solubilizing agent in isoelectric focusing applications is tested to meet low metal content specifications.

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent commonly used protein solubilization in isoelectric focusing and two-dimensional electrophoresis as well as used in breaking protein-protein interactions. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 1-4% (w/v) are typically used in an IEF gel. A commonly used IEF sample solution consists of 8M urea, 4% CHAPS, 50-100mM dithiothreitol (DTT), and 40mM Tris. Furthermore, its small micellar molecular weight (6150) and high critical micellar concentration (6-10mM) allow it to be removed from samples by dialysis.

The preparation of CHAPS is 50mg/ml. CHAPS is soluble in water.

Storage
Store at RT

Specification

Appearance White to pale white powder
Mol. Formula C32H58N2SO7
Mol. Weight 614.89
CAS RN 75621-03-3

 

Assay Specifications
Purity ≥99.0%
Loss on drying ≤4.0%
TLC One spot
Solubility (10% aq.) Clear, colorless solution

Ordering Information

Catalog No Description Pack Size
PC0925-5g 3-[(3-cholamidopropyl)dimethyl-ammonio]-1- propanesulfonate (CHAPS) 5g

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3-[(3-cholamidopropyl)dimethyl-ammonio]-1- propanesulfonate (CHAPS)

 

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