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Home Biochemical Biochemicals Deoxyribonuclease I (DNase I)

Deoxyribonuclease I (DNase I)

Grade
Molecular Biology Grade

Description
DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA and produce 3’-OH oligonucleotides in the presence of divalent cations. In the reaction solution, with the presence of Mg2+ helps the enzyme to produce nicks in double-stranded DNA, while with the presence of Mn2+, DNase I cleaves both strands of the DNA. DNase I is also useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA.

DNase I is offered as lyophilized powder with an activity of 500 Kunitz U/mg and is a chromatographically pure preparation. For greatest stability, DNase I is suggested to be dissolved at a concentration of at least 1mg/ml in 50% glycerol with 20mM Tris-HCl, pH7.5, and 1mM MgCl2. This solution can be stored at -20°C for at least a year.

Specification

Appearance White to off-white crystalline powder; a partially purified, lyophilized powder
Source Bovine pancreas All products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for exportation
Unit Definition One unit of DNase I is defined as the amount required to completely degrade 1µg of lambda DNA in 10 minutes at 37°C in 50µl of a buffer containing 40mM Tris-HCl (pH8.0), 2.5mM (up to 10mM) MgSO4, 1mM (up to 10mM) CaCl2. Under these assay conditions one unit of DNase activity is approximately equal to one Kunitz unit.

Storage
Store at -20°C

 

Assay Resuls
Activity >500Kunitz U/mg
Loss on Drying ≤5.0%
Residue on Ignition ≤1.0%

Ordering Information

Catalog No Description Pack Size
PC0704-25mg Deoxyribonuclease I (DNase I) 25mg
PC0704-50mg Deoxyribonuclease I (DNase I) 50mg
PC0704-100mg Deoxyribonuclease I (DNase I) 100mg
PC0704-1g Deoxyribonuclease I (DNase I) 1g

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Deoxyribonuclease I (DNase I)

 

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