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Home Biochemical Biochemicals Deoxyribonuclease I (DNase I)

Deoxyribonuclease I (DNase I)

Molecular Biology Grade

DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA and produce 3’-OH oligonucleotides in the presence of divalent cations. In the reaction solution, with the presence of Mg2+ helps the enzyme to produce nicks in double-stranded DNA, while with the presence of Mn2+, DNase I cleaves both strands of the DNA. DNase I is also useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA.

DNase I is offered as lyophilized powder with an activity of 500 Kunitz U/mg and is a chromatographically pure preparation. For greatest stability, DNase I is suggested to be dissolved at a concentration of at least 1mg/ml in 50% glycerol with 20mM Tris-HCl, pH7.5, and 1mM MgCl2. This solution can be stored at -20°C for at least a year.


Appearance White to off-white crystalline powder; a partially purified, lyophilized powder
Source Bovine pancreas All products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for exportation
Unit Definition One unit of DNase I is defined as the amount required to completely degrade 1µg of lambda DNA in 10 minutes at 37°C in 50µl of a buffer containing 40mM Tris-HCl (pH8.0), 2.5mM (up to 10mM) MgSO4, 1mM (up to 10mM) CaCl2. Under these assay conditions one unit of DNase activity is approximately equal to one Kunitz unit.

Store at -20°C


Assay Resuls
Activity >500Kunitz U/mg
Loss on Drying ≤5.0%
Residue on Ignition ≤1.0%

Ordering Information

Catalog No Description Pack Size
PC0704-25mg Deoxyribonuclease I (DNase I) 25mg
PC0704-50mg Deoxyribonuclease I (DNase I) 50mg
PC0704-100mg Deoxyribonuclease I (DNase I) 100mg
PC0704-1g Deoxyribonuclease I (DNase I) 1g


Deoxyribonuclease I (DNase I)


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