Vivantis Technologies Sdn Bhd

Grade
Molecular Biology Grade

Description
DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA and produce 3’-OH oligonucleotides in the presence of divalent cations. In the reaction solution, with the presence of Mg²⁺ helps the enzyme to produce nicks in double-stranded DNA, while with the presence of Mn2+, DNase I cleaves both strands of the DNA. DNase I is also useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving doublestranded DNA.

DNase I is offered as lyophilized powder with an activity of 500 Kunitz U/mg and is a chromatographically pure preparation. For greatest stability, DNase I is suggested to be dissolved at a concentration of at least 1mg/ml in 50% glycerol with 20mM Tris-HCl, pH7.5, and 1mM MgCl₂. This solution can be stored at - 20°C for at least a year

Source
Bovine pancreas All products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for exportation.

Unit Definition
One unit of DNase I is defined as the amount required to completely degrade 1µg of lambda DNA in 10 minutes at 37°C in 50µl of a buffer containing 40mM Tris-HCl (pH8.0), 2.5mM (up to 10mM) MgSO4, 1mM (up to 10mM) CaCl2. Under these assay conditions one unit of DNase activity is approximately equal to one Kunitz unit.

Specification

Storage
Store at -20°C

Ordering Information

Download
Manual

Deoxyribonuclease I (DNase I)

Download
MSDS

Deoxyribonuclease I (DNase I)

Publication

This Product Has Been Used In:

Eslahi M R, et al. (2021) cDNA-AFLP Analysis of Plant Defense Genes Expressed in Wheat (cv. Chamran) Infected with Mycosphaerella graminicola. JAST 2021; 23 (3) :699-710