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Home Biochemical Biochemicals Deoxyribonuclease I (DNase I) - For RNA work

Deoxyribonuclease I (DNase I) - For RNA work

Grade
Ultra Pure Grade

Description
DNase I is a recombinant form of DNase I which is RNase-free, originally isolated from bovine pancreas, is a recombinant enzyme expressed in Pichia pastoris. It is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides.

DNase I manufactured using state-of-the-art processes yielding animal-free material. The enzyme is highly purified and rigorously tested for contaminating RNase and protease activity of RT-PCR. It is an important tool for all applications requiring DNA-free RNA templates and achieving reliable results with undegraded and stable RNA.

Specification

Appearance Colorless to light yellow, clear solution.
Source Bovine pancreas.
Unit Definition One unit according to Kunitz produces an increase in absorbance of 0.001/minute under assay conditions in 1ml at 260nm.

Storage
Store at -20°C

 

Assay Resuls
Volume act. with calf thymys DNA (Kunitz) 11875 u/ml
Volume act. with calf thymys DNA (Kunitz) 993 KU/ml
Protease up to 50U w.casein resorufinm

Not detectable.

RNases up to 10U with MS II RNA

Not detectable.

Ordering Information

Catalog No Description Pack Size
PC0719 - 50kU Deoxyribonuclease I (DNase I) - For RNA work 50kU
PC0719 - 100kU Deoxyribonuclease I (DNase I) - For RNA work 100kU

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Deoxyribonuclease I (DNase I) - For RNA work

 

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